Conventional Versus Molecular Methods for Diagnosis of Burkholderia cepacia from Different Clinical Samples of Iraqi Patients

The study aims at isolating and identification of Burkholderia cepacia bacteria from clinical samples various pathological conditions such as diabetic foot ulcers, burn, wound, sputum urine. present includes 280 collected patients suffering ulcer, cystic fibrosis, burns, sputum, wounds who attend Alsader Medical City Al-Hakim General Hospital during the period extended September 2020 to February 2021 ( men women ) with age groups between (1 -75) years. bacterial isolates were detected by classical molecular technique (PCR), where frequency among males 213 (79.2%) was more than that in female 67 (20.8%). distribution is made according group; it appears high for following 26.8% group (31-45) years male female; 22% 7.6% (1-15) female. results revealed 42/80 specimens total number are using 16SrRNA gene differentiate B. other (G-) bacteria, 30/80 recA differentiated complex.